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JoVE Journal Biology

効率的な哺乳動物細胞発現および結晶化のための細胞外糖タンパク質のシングルステップの精製

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Journal (Biology)

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Isolation and Characterization of the Murine Uterosacral Ligaments and Pelvic Floor Organs

Journal (Biology)

Isolation and Characterization of the Murine Uterosacral Ligaments and Pelvic Floor Organs

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効率的な哺乳動物細胞発現および結晶化のための細胞外糖タンパク質のシングルステップの精製

Article DOI: 10.3791/53445-v • 07:08 min • December 23rd, 2015

December 23rd, 2015 •

Daniel L. Kober*^1,2,3, Zeynep Yurtsever*^2,3,4,5, Thomas J. Brett^2,3,5,6,7

¹Molecular Microbiology and Microbial Pathogenesis Program, Washington University School of Medicine, ²Department of Internal Medicine, Washington University School of Medicine, ³Drug Discovery Program in Pulmonary and Critical Care Medicine, Washington University School of Medicine, ⁴Biochemistry Program, Washington University School of Medicine, ⁵Center for the Investigation of Membrane Excitability Diseases, Washington University School of Medicine, ⁶Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, ⁷Department of Cell Biology and Physiology, Washington University School of Medicine

* These authors contributed equally

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Tags

生化学、106号、哺乳動物のタンパク質の発現、ニッケルアフィニティークロマトグラフィー、グリコシル化、高分子結晶

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