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Genetics

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JoVE Journal Genetics
RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing
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RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing

Article DOI: 10.3791/54280-v • 08:34 min • September 27th, 2016
September 27th, 2016 •
*1, *2, 2, 2, 2, 2
1Department of Ophthalmology, Saevit Eye Hospital, 2Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center
* These authors contributed equally

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Summary
Automatic Translation

Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.

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RNA Interference Heat Shock Protein 27 Corneal Epithelial Wound Healing Small Interfering RNA Messenger RNA RNA Degradation Pest Space Cellular Processes Skin Mucosa Bronchial Epithelium Growth Medium Confluence Transfection Re-agent Reduced Serum Medium SiRNA Scrambled Control SiRNA Nanomolar
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