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Bioengineering
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JoVE Journal Bioengineering
Matrices 2D y 3D para estudiar la formación y la actividad invadosoma lineal
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Matrices 2D y 3D para estudiar la formación y la actividad invadosoma lineal

Article DOI: 10.3791/54911
June 2nd, 2017 •
*1,2, *1, 1,3,4, 2, 2, 1, 1
1INSERM U1053, 2Icahn School of Medicine at Mount Sinai, Tisch Cancer Institute, 3Faculté de Médecine, Casablanca, Université Mohammed 6 des Sciences de la Santé (UM6SS), 4Laboratoire National de Référence
* These authors contributed equally

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June 2nd, 2017

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Este protocolo describe cómo preparar una matriz mixta 2D, que consta de gelatina y colágeno I, y un colágeno 3D para estudiar invadosomas lineales. Estos protocolos permiten el estudio de la formación invadosoma lineal, la actividad de degradación de la matriz, y la capacidad de invasión de células primarias y líneas celulares de cáncer.

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