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JoVE Journal Cancer Research
Counting Proteins in Single Cells with Addressable Droplet Microarrays
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Counting Proteins in Single Cells with Addressable Droplet Microarrays

Article DOI: 10.3791/56110-v • 12:25 min • July 6th, 2018
July 6th, 2018 •
*1, *1, 1, 1
1Institute of Chemical Biology, Department of Chemistry, Imperial College London
* These authors contributed equally

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Summary
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Here we present addressable droplet microarrays (ADMs), a droplet array based method able to determine absolute protein abundance in single cells. We demonstrate the capability of ADMs to characterize the heterogeneity in expression of the tumor suppressor protein p53 in a human cancer cell line.

Tags

Proteins Single Cells Addressable Droplet Microarrays Protein Copy Number Single-cell Resolution Gene Expression Heterogeneity Single-cell Assays Antibody Microarrays Fused Silica Tubing Concentric Nozzle
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