Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Dissection and Explant Culture of Murine…
JoVE Journal
Medicine

A subscription to JoVE is required to view this content.

JoVE Journal Medicine
Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment
Generation of a Rat Model of Acute Liver Failure by Combining 70% Partial Hepatectomy and Acetaminophen
Journal (Medicine)
Generation of a Rat Model of Acute Liver Failure by Combining 70% Partial Hepatectomy and Acetaminophen
Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs
Journal (Medicine)
Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs
 

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Article DOI: 10.3791/56712-v • 09:30 min • January 13th, 2018
January 13th, 2018 •
1,2, 1,2, 1,2
1Institute of Pharmacology and Toxicology, University of Würzburg, 2Rudolf Virchow Center for Experimental Biomedicine, University of Würzburg

Chapters

Summary
Automatic Translation

We describe an in vitro assay to model chorioallantoic attachment, the first step in placenta formation. The protocol demonstrates the dissection and explant culture of murine allantoides on immobilized α4β1 integrin. Allantois attachment is evaluated microscopically at pre-determined time points.

Tags

Dissection Explant Culture Murine Allantois In Vitro Analysis Allantoic Attachment Placenta Formation Mammalian Embryo Development Alpha 4/Beta 1 Integrin Solution Coating Incubation Supernatant Washing Medium Block Solution Pregnant Mouse
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter