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Immunology and Infection

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Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Article DOI: 10.3791/57196-v • 08:52 min • March 23rd, 2018
March 23rd, 2018 •
1, 1, 1,2, 1, 2, 1
1Department of Biological Sciences, Columbia University, 2Department of Biology, Barnard College, Columbia University

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Summary
Automatic Translation

We describe fixation, paraffin embedding, and thin sectioning techniques for microbial colony biofilms. In prepared samples, biofilm substructure and reporter expression patterns can be visualized by microscopy.

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Paraffin Embedding Thin Sectioning Microbial Colony Biofilms Microscopic Analysis Gene Expression Fluorescent Protein Production Native Morphologies Exopolymeric Substances Biofilm Architecture Downstream Treatments Staining High-resolution Imaging Agar Tryptone Solution Conical Tube Square Dish Solidify Lint-free Tissue Colony Biofilms P.Aeruginosa Colony Cell Suspension Medium Bi-layer Plate
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