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JoVE Journal Developmental Biology
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Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

Article DOI: 10.3791/57212-v • 07:18 min • April 19th, 2018
April 19th, 2018 •
*1, *2, 1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health
* These authors contributed equally

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Summary
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The precise identification of satellite cells is essential for studying their functions under various physiological and pathological conditions. This article presents a protocol to identify satellite cells on adult skeletal muscle sections by immunofluorescence-based staining.

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Skeletal Muscle Satellite Cells Immunofluorescence Pax7 Antibody Laminin Antibody Muscle Stem Cells Physiological Conditions Pathological Conditions Liquid Nitrogen Bath Methylbutane Optimal Cutting Temperature Compound (O.C.T.) Cryostat Sectioning Microcentrifuge Tube Specimen Hold
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