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Journal / Developmental Biology / CRISPR-medieret tab af funktion analyse i…
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Developmental Biology

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JoVE Journal Developmental Biology
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CRISPR-medieret tab af funktion analyse i cerebellare granula celler ved hjælp af In Utero elektroporation-baseret overførsel af gener

Article DOI: 10.3791/57311-v • 09:39 min • June 9th, 2018
June 9th, 2018 •
*1, *2, 2, 3,4,5, 1, 3,4
1Division of Molecular Neurogenetics, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 2Division of Molecular Genetics, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 3Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), 4Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 5Department of Pediatric Hematology and Oncology, Heidelberg University Hospital
* These authors contributed equally

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Konventionelle tab af funktion undersøgelser af gener ved hjælp af knockout dyr har ofte været dyrt og tidskrævende. Elektroporation-baseret CRISPR-medieret somatiske mutagenese er et kraftfuldt værktøj til at forstå genet fungerer in vivo. Her rapporterer vi en metode til at analysere knockout fænotyper i prolifererende celler af lillehjernen.

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Udviklingsmæssige biologi sag 136 målrettet gen levering i utero elektroporation lillehjernen GNP Cre CRISPR/Cas9 hjernens udvikling hjernetumorer
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