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JoVE Journal Cancer Research
Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation
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Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation

Article DOI: 10.3791/57329-v • 07:00 min • August 23rd, 2018
August 23rd, 2018 •
1, 2, 1, 3
1Department of Rheumatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 2Department of Nephrology and Rheumatology, Yongchuan Hospital of Chongqing Medical University, 3Department of Medical Oncology, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University

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Summary
Automatic Translation

Here, we present a reliable and straightforward two-dimensional (2D) coculture system for studying the interaction between tumor cells and bone marrow adipocytes, which reveals a dual effect of melanoma cell-derived factors on the bone marrow adipocytes differentiation and also poses a classic method for the mechanistic study of bone metastasis.

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Bone Metastasis Melanoma Cells Bone Marrow Adipocytes Cancer Microenvironment Crosstalk Osteoblasts B16F10 Melanoma Cells DMEM FBS Serum-free Culture Medium Conditioned Supernatant Centrifugation Sterile Container Conditioned Medium Bone Marrow Stromal Cells
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