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Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection
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Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection

Article DOI: 10.3791/57628-v • 08:50 min • May 13th, 2018
May 13th, 2018 •
*1, *1, 1,2
1Environmental and Life Sciences Graduate Department, Trent University, 2Forensic Science Department, Trent University
* These authors contributed equally

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Summary
Automatic Translation

Smut fungi cause many devastating agricultural diseases. They are dispersed as dormant teliospores that germinate in response to environmental cues. We outline two methods to investigate molecular changes during germination: measuring respiration increase to detect metabolic activation and assessing changing molecular events by isolating teliospores at distinct morphological stages.

Tags

Teliospore Germination Microrespiration Analysis Microdissection Gene Levels Disease Dispersal Transitions During Germination Gene Expression Control Respiration Level Physical Isolation Pathogen Dissemination Spore Germination Disease Spread Corncob Infection Teliospore Viability Germination Test Induce Germination Respiration Experiment Biosafety Cabinet Potato Dextrose Broth Streptomycin Sulfate Kanamycin Sulfate
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