Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / In Vitro Differentiation of Mouse…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device
Journal (Immunology and Infection)
Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device
Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
Journal (Immunology and Infection)
Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
 

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells

Article DOI: 10.3791/58087-v • 10:28 min • September 10th, 2018
September 10th, 2018 •
1,2,3, 3, 1,2
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine, National University of Singapore

Chapters

Summary
Automatic Translation

Here, we present a protocol to differentiate murine granulocyte-macrophage-colony-stimulating-factor-producing T helper (THGM) cells from naive CD4+ T cells, including isolation of naive CD4+ T cells, differentiation of THGM, and analysis of differentiated THGM cells. This method can be applied to studies of the regulation and function of THGM cells.

Tags

In Vitro Differentiation Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF) T Helper (THGM) Cells CD4 T-cell Field Subset Of T-helper Cells Inflammatory Diseases Multiple Sclerosis Technique GM-CSF Expressing T-cells Naive CD4 T-cells Gamma OI17 Expression Cells Cellular Source Regression And Function Demonstration Yi Lu Graduate Student Forceps Abdomen Incision Peritoneal Sac Spleen Cell Isolation Buffer Syringe Maceration Cell Collection Centrifugation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter