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A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

Article DOI: 10.3791/58160-v • 04:48 min • July 10th, 2018
July 10th, 2018 •
1, 1, 1
1Chemical Biology and Therapeutics, Novartis Institutes for BioMedical Research

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Summary
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The goal of this protocol is to facilitate the study of NMDA-receptors (NMDAR) at a larger scale and allow the examination of modulatory effects of small molecules and their therapeutic applications.

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High-throughput Calcium-flux Assay NMDA-receptors Sensitivity To Glycine/D-serine And Glutamate Neurological Diseases NMDA Modulators NMDA-receptor Activity Ligands Compounds Subtypes Specific In Biology NR1 NR2A Cells 384 Well Plate HEK 293 Cells Protective Compound Calcium 6 Dye Incubation Buffer
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