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Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Article DOI: 10.3791/58386-v • 08:38 min • October 4th, 2018
October 4th, 2018 •
1,2
1Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo, 2K. G. Jebsen Centre for B cell malignancies and K. G. Jebsen Centre for Cancer Immunotherapy, University of Oslo

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Summary
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Here, a protocol for medium- to high-throughput analysis of protein phosphorylation events at the cellular level is presented. Phospho flow cytometry is a powerful approach to characterize signaling aberrations, identify and validate biomarkers, and assess pharmacodynamics.

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Phospho Flow Cytometry Fluorescent Cell Barcoding Single Cell Signaling Analysis Biomarker Discovery Signaling Pathways Stimuli Drugs Medium To High Throughput Fashion RPMI 16/40 Medium 96 Well V-bottom Plate Fixed Buffer One Control Sample Anti-IgM Simulation Time Course Fix Plate Water Bath PBS Wash Barcoding Reagents
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