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Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

Article DOI: 10.3791/59191-v • 08:32 min • February 22nd, 2019
February 22nd, 2019 •
1, *1, *1, 1, 1
1Section of Pediatric Hematology-Oncology, Department of Pediatrics, University of Oklahoma Health Sciences Center
* These authors contributed equally

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Summary
Automatic Translation

Transgenic lck:eGFP zebrafish express GFP highly in T lymphocytes, and have been used to study T cell development and acute lymphoblastic leukemia. This line can be used to study B cells, which express lck at lower levels. This protocol describes purification of malignant and non-malignant B cells from lck:eGFP zebrafish.

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Isolate Malignant B Cells Non-malignant B Cells Lck:eGFP Zebrafish Study T Cells Technique B-ALL T-ALL Cells Fish With Cancer B Cell Development Adaptive Immune System Malignancies Pediatric Cancer Dissections Thymus Samples Marrow Samples Visual Demonstration Fluorous Anesthetizing Tricaine Epifluorescence Microscope GFP Filter Fluorescent Thymi Dorsal Medial Aspect Of The Branchial Cavity Petri Dish Fluorescent Microscope GFP Positive Thymi Brightfield Microscopy Settings Kidney Marrow Spleen Sorting Medium
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