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JoVE Journal Medicine
Assessment and Characterization of Hyaloid Vessels in Mice
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Assessment and Characterization of Hyaloid Vessels in Mice

Article DOI: 10.3791/59222-v • 08:22 min • May 15th, 2019
May 15th, 2019 •
1, 1, 1, 1,2
1Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, 2The Manton Center for Orphan Disease Research, Boston Children's Hospital, Harvard Medical School

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Summary
Automatic Translation

This protocol describes both in vivo and ex vivo methods to fully visualize and characterize hyaloid vessels, a model of vascular regression in mouse eyes, using optical coherence tomography and fundus fluorescein angiography for the live imaging and ex vivo isolation and subsequent flat mount of hyaloid for quantitative analysis.

Tags

Assessment Characterization Hyaloid Vessels Mice In Vivo Ex Vivo Visualization Experimental Model Vascular Regression Longitudinal Observation Quantification Dissected Whole Hyaloid Vessels Pathogenesis Basic Mechanisms Persistent Fetal Vasculature Cellular Mechanisms Molecular Mechanisms Regulation Ocular Vascular Regression Angiogenesis Research Hyaloid Isolation Steps Dissection Hands-on Information Technique Optical Coherence Tomography (OCT) Imaging Fundus Image Focus Angle OCT Imaging Software Fundus Fluorescein Angiography Imaging
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