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Journal / Genetics / Sequencing of mRNA from Whole Blood using…
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Genetics

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JoVE Journal Genetics
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Article DOI: 10.3791/59377-v • 11:26 min • June 3rd, 2019
June 3rd, 2019 •
1, 2, 3, 1
1Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, 2Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, 3Department of Animal Science, Njala University

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Nanopore sequencing is a novel technology that allows cost-effective sequencing in remote locations and resource-poor settings. Here, we present a protocol for sequencing of mRNAs from whole blood that is compatible with such conditions.

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Nanopore Sequencing MRNA Sequencing Whole Blood Sequencing Technology Portability Long Read Length Real-time Data Disease Outbreaks Remote Locations Ebola Virus Outbreaks MinION Device Library Preparation Flow Cell Loading Touchdown PCR CDNA Amplification Hot Start High Fidelity DNA Polymerase Reaction Buffer Template Magnetic Beads
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