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JoVE Journal Developmental Biology
Generatie van Organoids van muis Extrahepatic galwegen
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Generatie van Organoids van muis Extrahepatic galwegen

Article DOI: 10.3791/59544-v • 09:13 min • April 23rd, 2019
April 23rd, 2019 •
1, 1, 1,2, 1,2, 1
1Departments of Internal Medicine, The University of Michigan, 2Molecular & Integrative Physiology, The University of Michigan

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Dit protocol beschrijft de productie van een muis extrahepatic gal 3-dimensionale organoid kanaalsysteem. Deze biliaire organoids kan worden gehandhaafd in cultuur te bestuderen van cholangiocyte biologie. Biliaire organoids express markers van voorlopercellen zowel biliaire cellen en zijn samengesteld uit gepolariseerde epitheliale cellen.

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Ontwikkelingsbiologie kwestie 146 biliaire voorlopercellen cholangiocytes organoids 3-dimensionale cultuur systeem drug screening cholangiopathies
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