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Journal / Biochemistry / Measuring Interactions between Fluorescent Probes …
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Biochemistry

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JoVE Journal Biochemistry
Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
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Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Article DOI: 10.3791/59925-v • 07:15 min • January 2nd, 2020
January 2nd, 2020 •
1, 2, 2, 3
1PICT Platform, Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille

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Summary
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This protocol describes an original setup combining spectral and fluorescence lifetime measurements to evaluate Förster resonance energy transfer (FRET) between rhodamine-based fluorescent probes and lignin polymer directly in thick plant sections.

Tags

Fluorescent Probes Lignin Plant Sections SFLIM Autofluorescence FRET Analysis Nano Scale Biomass Sectioning Microtome Gentle Washings Fluorescent Lifetime Spectrum Measurements FRET Determination TAG Molecules In Situ Wood Samples Wheat Samples Fiber Samples Fragment Samples Razor Blades Vacuum PEG Concentrations
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