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Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization

Article DOI: 10.3791/60458-v • 09:50 min • October 18th, 2019
October 18th, 2019 •
*1, *1, 1, 2, 1
1Department of Biological Sciences, University of Texas at Dallas, 2Department of Urology, University of Texas Southwestern Medical Center
* These authors contributed equally

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Summary
Automatic Translation

This protocol is for the unbiased detection of tissue-associated bacteria in patient biopsies by 16S rRNA in situ hybridization and confocal microscopy.

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Tissue-resident Bacteria Bladder Biopsies 16S RRNA Fluorescence In Situ Hybridization Detection Technique Unbiased Detection Bacterial Species Recurrent Urinary Tract Infection Tissue-penetrating Antibiotics Resident Bacterial Populations Coverslip Placement Imaging Optimization Jashkaran Gadhvi Fume Hood Biosafety Cabinet Buffers Reagents Coplin Jars
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