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Quantification of Proliferative and Dead Cells in Enteroids
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Quantification of Proliferative and Dead Cells in Enteroids

Article DOI: 10.3791/60501-v • 10:47 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1, 1, 1, 1, 2, 2,3, 1, 1, 1
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3Department of Pathology, Brigham and Women's Hospital–Harvard Medical School
* These authors contributed equally

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Summary
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The presented protocol uses flow cytometry to quantify the number of proliferating and dead cells in cultured mouse enteroids. This method is helpful to evaluate the effects of drug treatment on organoid proliferation and survival.

Tags

Enteroid Isolation Cell Proliferation Cell Viability Drug Testing Inflammatory Cytokines Water Therapy Inflammatory Bowel Disease Therapeutical Cytokines Organoids Intestinal Crypts Enteroid Culture Ileum Dissection DPBS (Dulbecco's Phosphate-Buffered Saline) Buffer One Buffer Two
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