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Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

Article DOI: 10.3791/60989-v • 09:40 min • April 2nd, 2020
April 2nd, 2020 •
1, 1, 1, 1
1Program in Behavioral Neuroscience, Department of Psychology, University of South Carolina

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We present a protocol to label and analyze pyramidal neurons, which is critical for evaluating potential morphological alterations in neurons and dendritic spines that may underlie neurochemical and behavioral abnormalities.

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Ballistic Labeling Pyramidal Neurons Brain Slices Primary Cell Culture Morphological Alterations Neurochemical Abnormalities Behavioral Abnormalities Visualizing Neurons Brain Regions Rats Reconstruction Software Neurocognitive Dysfunction PVP Solution Tungsten Microcarrier Beads Methylene Chloride Lipophilic DilC18(3)dye Glass Slide Air Dry Centrifuge Tubes Water Bath Sonicate
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