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Developmental Biology

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JoVE Journal Developmental Biology
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
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Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

Article DOI: 10.3791/61034-v • 10:14 min • February 27th, 2021
February 27th, 2021 •
1,2, 3, 1,2
1Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, 2Laboratório Associado para a Química Verde, 3Dipartimento di Bioscienze, Universitá degli Studi di Milano

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Summary
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This protocol describes in detail how the plant material for immunolocalization of Arabinogalactan proteins and pectins is fixed, embedded in a hydrophilic acrylic resin, sectioned and mounted on glass slides. We show cell wall related epitopes will be detected with specific antibodies.

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Fluorescent Immunolocalization Arabinogalactan Proteins Pectins Cell Wall Plant Tissues Experiment Distribution Abundance Method Plant Tissue Sample Fixative Solution Trim Samples Immersing In Fixative Solution Vacuum Chamber Pressure Sinking Process Refrigerate
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