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A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
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A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Article DOI: 10.3791/61160-v • 06:50 min • May 29th, 2020
May 29th, 2020 •
*1,2, *1,3, 1, 1, 1
1Barbara Davis Center for Diabetes, University of Colorado School of Medicine, 2Department of Endocrinology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, 3Department of endocrinology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology
* These authors contributed equally

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Summary
Automatic Translation

We model a simple multiplexed ECL assay that combines 7 autoantibody assays together. The assay is capable of screening for T1D and multiple other autoimmune diseases, simultaneously, including celiac disease, autoimmune thyroid disease, and autoimmune polyglandular syndrome 1.

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High-Throughput Electrochemiluminescence 7-Plex Assay Type 1 Diabetes Multiple Autoimmune Diseases Autoimmune Cryo Disease Celiac Disease Autoimmune Polyglandular Syndrome-1 ECR Assay Antibody Assays Mixed Linker-coupled Antigen Solution Biotinylated Antigens STREPTAVIDIN Conjugated Linker Biotinylated Proteins Stop Solution Incubation
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