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Immunology and Infection

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Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Article DOI: 10.3791/61551-v • 09:35 min • April 20th, 2021
April 20th, 2021 •
1, 2, 1, 1
1Department of Biology, The City College of New York and The Graduate Center of The City University of New York, 2Department of Microbiology, Icahn School of Medicine at Mount Sinai

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Summary
Automatic Translation

Presented here is an assay to quantify somatic hypermutation within the immunoglobulin heavy chain gene locus using germinal center B cells from mouse Peyer’s patches.

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Somatic Hypermutation JH4 Intron Germinal Center B Cells Mouse Peyer's Patches Immune System Antibodies Pathogens Commensal Microbes Molecular Pathways Immunoglobulin Gene Diversification Genetically Engineered Mice PCR Sanger Sequencing Single Cell Sorting Deep Sequencing Dissection Method Visual Demonstration Abdominal Incision Peritoneal Cavity Small Intestine Connective Tissue Fat Tissue Peyer's Patches Identification
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