Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Generation of Human Neurons and Oligodendrocytes…
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions
Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro
Journal (Neuroscience)
Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro
Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)
Journal (Neuroscience)
Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)
 

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

Article DOI: 10.3791/61778-v • 10:53 min • November 9th, 2020
November 9th, 2020 •
*1, *1,2, *3, 1, 1, 3, 3,4, 1,5,6
1Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 2Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, 3Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 4Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 5Department of Neurology, Warren Alpert Medical School of Brown University, 6Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University
* These authors contributed equally

Chapters

Summary
Automatic Translation

The neuron-glial interactions in neurodegeneration are not well understood due to inadequate tools and methods. Here, we describe optimized protocols to obtain induced neurons, oligodendrocyte precursor cells, and oligodendrocytes from human pluripotent stem cells and provide examples of the values of these methods in understanding cell-type-specific contributions in Alzheimer’s disease.

Tags

Human Neurons Oligodendrocytes Pluripotent Stem Cells Neuron-oligodendrocyte Interactions Oligodendroglial Defects Neurodegeneration Functional Human Oligodendrocyte Lineage Cells Co-culture System Alzheimer's Disease Plate HEK293T Cells Transfection Viral Particles Purification H1 Human ES Cells Neuron Induction
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter