JoVE Journal
Biochemistry
Biochemistry
A subscription to JoVE is required to view this content.
Chapters
Summary
This protocol describes the removal of endogenous lipids from allergens, and their replacement with user-specified ligands through reverse-phase HPLC coupled with thermal annealing. 31P-NMR and circular dichroism allow for the rapid confirmation of ligand removal/loading, and the recovery of native allergen structure.