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Journal / Biochemistry / Spotvariation Fluorescenskorrelationsspektroskopi …
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Biochemistry

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JoVE Journal Biochemistry
Spotvariation Fluorescenskorrelationsspektroskopi för analys av molekylär diffusion vid plasmamembranet i levande celler
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Spotvariation Fluorescenskorrelationsspektroskopi för analys av molekylär diffusion vid plasmamembranet i levande celler

Article DOI: 10.3791/61823-v • 05:56 min • November 12th, 2020
November 12th, 2020 •
*1, *2, *1, 3, 4, 2, 1, 2
1CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, 2Faculty of Biotechnology, University of Wroclaw, 3Faculty of Biological Sciences, University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ
* These authors contributed equally

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Denna artikel syftar till att presentera ett protokoll om hur man bygger ett punktvariationsfluorescens korrelationsspektroskopi (svFCS) mikroskop för att mäta molekylär diffusion vid plasmamembranet i levande celler.

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Biokemi utgåva 165 punktvariation Fluorescenskorrelationsspektroskopi (svFCS) lateral diffusion plasmamembrandynamik molekylär inneslutning lipidnanodomäner cytoskelettnätverk diffusionslag
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