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Journal / Biochemistry / Frazionamento cellulare di cellule U937 mediante…
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Biochemistry

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JoVE Journal Biochemistry
Frazionamento cellulare di cellule U937 mediante purificazione del gradiente di densità isopicnica
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Frazionamento cellulare di cellule U937 mediante purificazione del gradiente di densità isopicnica

Article DOI: 10.3791/62119-v • 10:19 min • August 12th, 2021
August 12th, 2021 •
1, 1, 1, 1, 1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

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Questo protocollo di frazionamento consentirà ai ricercatori di isolare proteine citoplasmatiche, nucleari, mitocondriali e di membrana da cellule di mammifero. Le ultime due frazioni subcellulari vengono ulteriormente purificate tramite gradiente di densità isopicnica.

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Biochimica Numero 174 Frazionamento cellulare Nucleare Mitocondri Membrana Citoplasma Organelli Ultracentrifugazione Gradiente di densità U937
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