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Journal / Biochemistry / Fraccionamiento celular de células U937 por…
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JoVE Journal Biochemistry
Fraccionamiento celular de células U937 por purificación de gradiente de densidad isopícnica
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Fraccionamiento celular de células U937 por purificación de gradiente de densidad isopícnica

Article DOI: 10.3791/62119-v • 10:19 min • August 12th, 2021
August 12th, 2021 •
1, 1, 1, 1, 1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

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Este protocolo de fraccionamiento permitirá a los investigadores aislar proteínas citoplasmáticas, nucleares, mitocondriales y de membrana de células de mamíferos. Las dos últimas fracciones subcelulares se purifican aún más a través del gradiente de densidad isopícnica.

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Bioquímica Número 174 Fraccionamiento celular Nuclear Mitocondrias Membrana Citoplasma Orgánulos Ultracentrifugación Gradiente de densidad U937
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