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Immunology and Infection

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JoVE Journal Immunology and Infection
Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
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Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Article DOI: 10.3791/62154-v • 06:37 min • February 5th, 2021
February 5th, 2021 •
1, 1, 1, 1, 1
1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California

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This article outlines a detailed protocol for using the RNA-targeting Cas13D enzyme (RfxCas13D) in flies.

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CRISPR/CasRx RNA Targeting Drosophila Melanogaster Ubiquitous Gene Reduction Tissue-specific Gene Reduction Fruit Flies CRISPR-based Gene Reduction Two Component CasRx System GRNA Line Ubiq CasRx CurlyO Vial Dry Yeast Powder F1 Generation Carbon Dioxide Anesthesia Fluorescent Stereo Microscope Phenotypes Fluorescent Signal Expression Mendelian Genetics
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