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Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
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Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Article DOI: 10.3791/62272-v • 06:53 min • July 10th, 2021
July 10th, 2021 •
1, 1, 1, 1, 1
1Department of Veterinary Medicine, University of Sassari

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The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. Oocytes are vitrified at the MII stage using Cryotop to ensure the minimum essential volume.

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Vitrification In Vitro Matured Oocytes Adult Ovaries Prepubertal Ovaries Sheep Oocyte Preservation Genetic Selection Programs Biodiversity Conservation Biotechnology Research Ultrarapid Cooling Warming Cattle Oocyte Preservation Vitrification Protocol In Vitro Maturation Cryopreservation Trehalose Supplementation Denudation Of Cumulus Cells
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