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Biochemistry

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JoVE Journal Biochemistry
Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
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Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

Article DOI: 10.3791/62364-v • 10:32 min • April 23rd, 2021
April 23rd, 2021 •
1, 1, 1, 1, 1, 1, 1
1Diamond Light Source Ltd, Harwell Science & Innovation Campus

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Here, we present a protocol for cryogenic sample preparation and transfer of crystals into the vacuum endstation on beamline I23 at Diamond Light Source, for long-wavelength macromolecular X-ray crystallography experiments.

Tags

Sample Preparation Transfer Protocol In-Vacuum Long-Wavelength Crystallography Beamline I23 Diamond Light Source Macromolecular Crystallography Anomalous Signal Light Atoms Crystallographic Face Problem Identity And Location Determination Synchrotron Instrument Long Wavelengths Absorption Edges High Biological Relevance Elements Cryogenic Temperatures Vacuum Environment Cryo-electron Microscopy Sample Holders CombiPuck
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