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JoVE Journal Bioengineering
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In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

Article DOI: 10.3791/62497-v • 07:32 min • September 25th, 2021
September 25th, 2021 •
*1,2, *1, 1,5, 3,4, 3,4, 1,5,6
1Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Veterinary Pharmacology and Stem Cell Research Laboratory, Faculty of Veterinary Science, Chulalongkorn University, 2Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, 3Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, 4Center of Excellence in Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, 5Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, 6Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University
* These authors contributed equally

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Summary
Automatic Translation

This protocol presents a comparison between two different induction protocols for differentiating human dental pulp stem cells (hDPSCs) toward pancreatic lineages in vitro: the integrative protocol and the non-integrative protocol. The integrative protocol generates more insulin producing cells (IPCs).

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In Vitro Induction Human Dental Pulp Stem Cells Pancreatic Lineages Alternative Treatment Diabetes Type I Insulin Producing Cells Transplantation Integrative Induction Protocol Non-integrative Induction Protocol Natural Pancreatic Developmental Process Mesenchymal Stem Cells Multi-step Induction Protocol Definitive Endoderm Pancreatic Endoderm Pancreatic Endocrine Pancreatic Beta-cell Insulin Producing Cells Three Step Induction Approach PDX-1 Transcription Factor Differentiation Protocol
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