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JoVE Journal Genetics
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Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Article DOI: 10.3791/62598-v • 14:56 min • May 6th, 2022
May 6th, 2022 •
*1, *1, 1, 2,3, 1, 4, 5, 1, 1, 6, 7, 8, 9, 6
1Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, 2Centre for Computational Biology, University of Birmingham, 3Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, 4Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine, The University of Edinburgh, 5Ribeirão Preto School of Physical Education and Sport, University of São Paulo, 6Health Sciences Department, Ribeirao Preto Medical School, University of Sao Paulo, 7Faculty of Pharmaceutical Sciences, University of São Paulo, 8Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, 9Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo
* These authors contributed equally

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Summary
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The present study describes the workflow to manage DNA methylation data obtained by microarray technologies. The protocol demonstrates steps from sample preparation to data analysis. All procedures are described in detail, and the video shows the significant steps.

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DNA Methylation Association Strategy Obesity Omics Technologies Epigenetics Standardize Protocols Pipeline Bisulfite Treatment Denaturing DNA Bisphosphate Thermal Cycler Washing Steps Amplification Methylation Assay Hybridization Oven Sodium Hydroxide Solution Plastic Seal Vortexing RPM MSM
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