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Bioengineering

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JoVE Journal Bioengineering
Syntese og karakterisering af mRNA-Loaded Poly (Beta Aminoesters) Nanopartikler til vaccinationsformål
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Syntese og karakterisering af mRNA-Loaded Poly (Beta Aminoesters) Nanopartikler til vaccinationsformål

Article DOI: 10.3791/62889-v • 08:27 min • August 13th, 2021
August 13th, 2021 •
1, 1, 1, 1, 1, 1, 1, 1
1Grup d’Enginyeria de Materials (Gemat), Institut Químic de Sarrià (IQS), Universitat Ramon Llull (URL)

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Her præsenteres en simpel protokol til fremstilling af mRNA nanopartikler baseret på poly (beta aminoester) polymerer, let at skræddersy ved at ændre den indkapslede mRNA. Arbejdsgangen for syntetisering af polymerer, nanopartikler, og deres in vitro væsentlige karakterisering er også beskrevet. Der tilføjes også et proof-of-concept vedrørende immunisering.

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Bioengineering Udgave 174 mRNA-belastning poly(beta aminoester) polymerer polymere nanopartikler frysetørring vaccination
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