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Journal / Biochemistry / Shotgun Proteomics prøvebehandling automatiseret…
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Biochemistry

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JoVE Journal Biochemistry
Shotgun Proteomics prøvebehandling automatiseret af en open source lab robot
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
Journal (Biochemistry)
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
Journal (Biochemistry)
Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
 
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Shotgun Proteomics prøvebehandling automatiseret af en open source lab robot

Article DOI: 10.3791/63092-v • 10:12 min • October 28th, 2021
October 28th, 2021 •
1,3, 1,3, 1,3, 1,3, 1,2,3
1Department of Medicine-Cardiology, University of Colorado Anschutz Medical Campus, 2Department of Biochemistry & Molecular Genetics, University of Colorado Anschutz Medical Campus, 3Consortium for Fibrosis Research & Translation, School of Medicine, University of Colorado Anschutz Medical Campus

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Detaljeret protokol og tre Python scripts er fastsat for drift af en open source robot væske håndtering system til at udføre semi-automatiseret protein prøve forberedelse til massespektrometri eksperimenter, der dækker fjernelse af vaskemiddel, protein fordøjelse, og peptid afsaltning trin.

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