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Biochemistry

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JoVE Journal Biochemistry
Hagle proteomics prøvebehandling automatisert av en åpen kildekode lab robot
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Hagle proteomics prøvebehandling automatisert av en åpen kildekode lab robot

Article DOI: 10.3791/63092-v • 10:12 min • October 28th, 2021
October 28th, 2021 •
1,3, 1,3, 1,3, 1,3, 1,2,3
1Department of Medicine-Cardiology, University of Colorado Anschutz Medical Campus, 2Department of Biochemistry & Molecular Genetics, University of Colorado Anschutz Medical Campus, 3Consortium for Fibrosis Research & Translation, School of Medicine, University of Colorado Anschutz Medical Campus

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Detaljert protokoll og tre Python-skript er gitt for å betjene et åpen kildekode robotisert væskehåndteringssystem for å utføre halvautomatisk proteinprøvepreparering for massespektrometriforsøk, som dekker fjerning av vaskemiddel, protein fordøyelse og peptidavsaltingstrinn.

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