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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisering af inflammatorisk caspases induceret nærhed i humane monocytafledte makrofager
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Visualisering af inflammatorisk caspases induceret nærhed i humane monocytafledte makrofager

Article DOI: 10.3791/63162-v • 08:41 min • April 6th, 2022
April 6th, 2022 •
1, 1
1Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology, Baylor College of Medicine

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Denne protokol beskriver arbejdsgangen for at opnå monocytterafledte makrofager (MDM) fra humane blodprøver, en simpel metode til effektivt at introducere inflammatorisk caspase Bimolekylær Fluorescenskomplementering (BiFC) journalister i human MDM uden at gå på kompromis med cellelevedygtighed og adfærd og en billeddannelsesbaseret tilgang til måling af inflammatorisk caspaseaktivering i levende celler.

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Immunologi og infektion udgave 182
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