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Journal / Immunology and Infection / Visualisering av inflammatoriske caspaser…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisering av inflammatoriske caspaser indusert nærhet i humane monocytt-avledede makrofager
Isolation and Transplantation of Different Aged Murine Thymic Grafts.
Journal (Immunology and Infection)
Isolation and Transplantation of Different Aged Murine Thymic Grafts.
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
Journal (Immunology and Infection)
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
 
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Visualisering av inflammatoriske caspaser indusert nærhet i humane monocytt-avledede makrofager

Article DOI: 10.3791/63162-v • 08:41 min • April 6th, 2022
April 6th, 2022 •
1, 1
1Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology, Baylor College of Medicine

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Denne protokollen beskriver arbeidsflyten for å oppnå monocytter-avledede makrofager (MDM) fra menneskelige blodprøver, en enkel metode for effektivt å introdusere inflammatorisk caspase Bimolecular Fluorescence Complementation (BiFC) reportere i human MDM uten å gå på kompromiss med cellens levedyktighet og oppførsel, og en bildebasert tilnærming for å måle inflammatorisk caspase-aktivering i levende celler.

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Immunologi og infeksjon utgave 182
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