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Journal / Immunology and Infection / Odling av lymfocyter i simulerad mikrogravitation …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Odling av lymfocyter i simulerad mikrogravitation med hjälp av ett roterande cellodlingssystem
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Odling av lymfocyter i simulerad mikrogravitation med hjälp av ett roterande cellodlingssystem

Article DOI: 10.3791/63296-v • 09:28 min • August 25th, 2022
August 25th, 2022 •
1,2, 1,2,4, 1,3
1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 2Krembil Research Institute, Toronto Western Hospital, University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital, Sinai Health Systems, 4Medical Oncology and Hematology, Princess Margaret Cancer Centre

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Detta är en steg-för-steg-guide för att använda ett kommersiellt tillgängligt roterande cellodlingssystem för att odla lymfocyter i simulerad mikrogravitation med hjälp av specialiserade engångsodlingskärl. Denna odlingsmetod kan tillämpas på vilken cellodling som helst av suspensionstyp.

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Retraktion utgåva 186 suspensionscellodling lymfocyter simulerad mikrogravitation tidsmedelvärde av gravitationsvektorns nollifiering 2D-klinostat roterande cellodlingssystem roterande väggkärlanordning
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