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Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis

Article DOI: 10.3791/63346-v • 08:15 min • February 3rd, 2022
February 3rd, 2022 •
1, 1, 1, 2, 3
1School of Biosciences, University of Birmingham, 2Nanomedicine, Drug Delivery & Nanotoxicology Lab, School of Pharmacy, University of Birmingham, 3Mitochondrial Profiling Centre, University of Birmingham

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Summary
Automatic Translation

These protocols will help users probe mitochondrial energy metabolism in 3D cancer cell-line-derived spheroids using Seahorse extracellular flux analysis.

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Mitochondrial Energy Metabolism 3D Microtissue Spheroids Extracellular Flux Analysis Cell Lines Phenotypes Drug Treatments Field Drug Discovery Cancer Research Physiological Model Cell Monolayer Experiments Vitreous Spheroids In Vivo Tumors Carbohydrate Utility Seahorse Extracellular Flux 96 Instrument Spheroid Viability Inverted Light Microscope Phase Contrast Sensor Cartridge Calibrant
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