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JoVE Journal Bioengineering
Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
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Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Article DOI: 10.3791/63531-v • 08:25 min • April 6th, 2022
April 6th, 2022 •
1, 2, 1, 1, 1, 3,4, 1
1Department of Biotechnology, Ming Chuan University, 2Department of Tourism and Leisure, Hsing Wu University, 3Department of Science Education and Application, National Taichung University of Education, 4Department of Soil and Environmental Sciences, National Chung Hsing University

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Summary
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Here, we present a protocol to inactivate pathogenic bacteria with reactive oxygen species produced during photolysis of flavin mononucleotide (FMN) under blue and violet light irradiation of low intensity. FMN photolysis is demonstrated to be a simple and safe method for sanitary processes.

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Inactivation Pathogens Visible-light Photolysis Riboflavin-5'-phosphate Flavin Mononucleotide Free Radicals Staphylococcus Aureus E.coli Photosensitizer Violet Light Skin Therapy Subcutaneous Tissues Optical Fiber Food Industry Sanitation Light-emitting Diodes Opaque Plastic Cup Glass Test Tube Reactants Constant Temperature Infrared Thermometer FMN Solution Potassium Phosphate Buffer
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