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JoVE Journal Bioengineering
Formulation et caractérisation d’un système de transport de dopamine à base d’exosomes
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Formulation et caractérisation d’un système de transport de dopamine à base d’exosomes

Article DOI: 10.3791/63624-v • 06:08 min • April 4th, 2022
April 4th, 2022 •
1,2, 3,4,5,6, 2,7, 2, 2, 8, 8, 3,4,5,6,9
1Vocational School of Health Services/İstanbul, Altinbas University, 2Chemical Metallurgy Faculty, Department of Bioengineering, Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology, Istinye University, 43D Bioprinting Design & Prototyping R&D Center, Istinye University, 5Stem Cell and Tissue Engineering R&D Center, Istinye University, 6Medicine Faculty, Istinye University, 7Vocational School of Health Services, Istinye University, 8The V. Akhundov National Scientific Research Medical Prophylactic Institute, 9Center for Stem Cells and Regenerative Medicine (LivMedCell), Liv Hospital

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Ici, nous avons cherché à obtenir une formulation par chargement dopaminergique des exosomes isolés de cellules souches issues des cellules souches mésenchymateuses gelées de Wharton. L’isolement et la caractérisation des exosomes, la charge du médicament dans les exosomes résultants et l’activité cytotoxique de la formulation développée sont décrits dans ce protocole.

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Système de transport de dopamine à base d’exosomes vésicules extracellulaires cargaison intercellulaire communication fluides corporels barrière hémato-encéphalique système nanoporteur dopamine encapsulante cellules souches mésenchymateuses de gelée de Wharton libération de médicaments tests de cytotoxicité viabilité des fibroblastes
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