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Journal / Neuroscience / Ex Vivo Release of Calcitonin Gene-Related…
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Neuroscience

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JoVE Journal Neuroscience
Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
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Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

Article DOI: 10.3791/63723-v • 08:39 min • May 16th, 2022
May 16th, 2022 •
1, 1, 1,2,3, 1
1Danish Headache Center, Department of Neurology, Rigshospitalet, University of Copenhagen, 2EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail), Univ Rennes, Inserm, 3Department of Biology, Section of Cell Biology and Physiology, University of Copenhagen

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Summary
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The present protocol describes the ex vivo calcitonin gene-related peptide (CGRP) release model and the strategy to quantify the effect of pharmacological agents on the amount of CGRP released from the trigeminovascular system in rodents.

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Ex Vivo Release Calcitonin Gene-Related Peptide Trigeminovascular System Rodents Investigate Mechanisms CGRP Release Trigeminal Vascular System Distinct Sites Rat Tissue Remove Skin Remove Muscle Separate Lower Jaws Expose Spinal Cord Expose Brainstem Cut Cranium Remove Bone Structures Expose Cerebellum Isolate TNC SIF Immersion Divide Cranium Remove Brain Isolate TG Mandibular Branch
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