Molecular and Immunologic Techniques in a Genetically Engineered Mouse Model of Gastrointestinal Stromal Tumor

Journal / Cancer Research /

JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content.

JoVE Journal Cancer Research

Article DOI: 10.3791/63853-v • 07:21 min • May 2nd, 2022

May 2nd, 2022 •

The goal of this manuscript is to describe the Kit^V558Δ/+ mouse model and techniques for successful dissection and processing of mouse specimens.

Molecular Techniques Immunologic Techniques Genetically Engineered Mouse Model Gastrointestinal Stromal Tumor Breeding Maintenance Dissection Specimen Processing Immunocompetent Mouse Model Translational Research Targeted Molecular Therapy Tyrosine Kinase Inhibitors Immunotherapies Sterilize Instruments Gloves Sterile Field Incision Abdominal Cavity Intra-abdominal Adhesions Draining Mesenteric Lymph Node Cecum Mesenteric Lymph Node Dissection Protein Isolation Histology Single Cell Suspension RPMI Medium GIST Isolation