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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Article DOI: 10.3791/63940-v • 08:45 min • April 21st, 2022
April 21st, 2022 •
1,2, 1,2, 1,2
1Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, 2Department of Chemistry, University of Illinois Urbana-Champaign

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Summary
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Post-transcriptional modifications of RNA represent an understudied layer of translation regulation that has recently been linked to central nervous system plasticity. Here, sample preparation and liquid chromatography-tandem mass spectrometry approach is described for simultaneous characterization of numerous RNA modifications in single neurons.

Tags

RNA Modifications Single Neurons Mass Spectrometry Profiling Post-transcriptional Regulation Sample Preparation Liquid Chromatography Tandem Mass Spectrometry Modified Nucleosides Chemical Profiles RNA Profiles Ganglia Preparation Protease Treatments Aplysia Californica
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