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Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
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Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Article DOI: 10.3791/63990-v • 09:57 min • October 17th, 2022
October 17th, 2022 •
*1,2, *1,3, 1,2, 1,4, 1,5, 1, 2, 1,6, 1
1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 3Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, 4School of Basic Medicine, The Fourth Military Medical University, 5College of Life Science, Northwest University, 6Xi'an Institute of Tissue Engineering and Regenerative Medicine
* These authors contributed equally

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Summary
Automatic Translation

The present protocol describes a method to extract extracellular vesicles from the peripheral blood and solid tissues with subsequent profiling of surface antigens and protein cargos.

Tags

Isolation Analysis Traceable Extracellular Vesicles Functionalized Extracellular Vesicles Plasma Solid Tissues Protocol Isolate Collect Protein Contents Functional Experiments High Yield Low Contamination Surface Antigens Protein Cargoes Physiological Studies Pathological Studies Diagnosis Of Cancers Inflammatory Diseases Osteoporosis Detection Of Surface Markers Flow Cytometer Quantity Of Tissue Extracellular Vesicle Concentration Dilution Maxillary Bone Samples Ophthalmic Tweezers Scissors Wash With PBS Liberase Incubate At 37 Degrees Celsius Centrifuge Sample
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