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Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
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Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Article DOI: 10.3791/64020-v • 12:27 min • July 26th, 2022
July 26th, 2022 •
*1, *1, *1, *1, *2, *2, *3, *1
1NanoFCM Co., Ltd, 2School of Sport, Exercise and Health Sciences, Loughborough University, 3Biomolecular Sciences Research Centre, Sheffield Hallam University
* These authors contributed equally

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Summary
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The latest generation of EV characterization tools are capable of single EV analysis across multiple parameters simultaneously. Nano-flow cytometry measures all biological particles larger than 45 nm without labeling and identifies specific characteristics of subpopulations by a variety of fluorescent labeling techniques.

Tags

Extracellular Vesicles Transmembrane Protein Characterization Nano-flow Cytometry EV Subpopulations Label-free Fluorescence False Positives Plasma CSF Culture Medium Viruses Lipid Nanoparticles Nanomaterials Labeling Protocol Fluorescence Optimization
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