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JoVE Journal Neuroscience
Culturas organotípicas de explantes da retina de macacos
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Culturas organotípicas de explantes da retina de macacos

Article DOI: 10.3791/64178-v • 10:32 min • August 24th, 2022
August 24th, 2022 •
1, 1, 1, 1, 2, 1
1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University, Yunnan University, 2Institute for Ophthalmic Research, Eberhard-Karls-Universität Tübingen

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Explantes retinianos obtidos de macacos selvagens foram cultivados in vitro. A degeneração retiniana e a via de sinalização GMPc-PKG foram induzidas com o uso do inibidor de PDE6 zaprinast. O acúmulo de GMPc nos explantes em diferentes concentrações de zaprinast foi verificado por imunofluorescência.

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Neurociência Edição 186
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