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Journal / Immunology and Infection / عزل براز الفئران وزرع…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
عزل براز الفئران وزرع الجراثيم
Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model
Journal (Immunology and Infection)
Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
Journal (Immunology and Infection)
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
 
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عزل براز الفئران وزرع الجراثيم

Article DOI: 10.3791/64310-v • 07:32 min • May 26th, 2023
May 26th, 2023 •
1, 2,3, 2, 1
1Division of Clinical Pharmacology, Department of Medicine Vanderbilt University Medical Center and Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Division of Pediatric Nephrology Department of Pediatrics, Vanderbilt University Medical Center, 3Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center

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Click here for the English version.

الهدف هنا هو تحديد بروتوكول للتحقيق في آليات dysbiosis في أمراض القلب والأوعية الدموية. تناقش هذه الورقة كيفية جمع عينات براز الفئران وزرعها بشكل معقم ، وعزل الأمعاء ، واستخدام طريقة "Swiss-roll" ، تليها تقنيات التلوين المناعي لاستجواب التغيرات في الجهاز الهضمي.

Tags

علم المناعة والعدوى ، العدد 195 ، زرع الجراثيم البرازية ، لفة سويسرية ، القلب والأوعية الدموية
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